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plko 1 trc  (Addgene inc)


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    Structured Review

    Addgene inc plko 1 trc
    Plko 1 Trc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1296 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plko+1+trc+cloning+plasmid/pLKO%2E1+-+TRC+cloning+vector+(Plasmid+%2310878)/pmc12898032-213-8-19
    Average 96 stars, based on 1296 article reviews
    plko 1 trc - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Cloning:

    Article Title: An extensive program of periodic alternative splicing linked to cell cycle progression
    Article Snippet: The Myc-His-Ubiquitin expression vector is a gift from Dr. Gary Johnson’s lab, and the Histone H2B-GFP expression vector is gift from Dr. Angelique Whitehurst’s lab. Plasmid transfections were performed using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol. .. The lentiviral vectors of shRNAs were obtained from Addgene in pLKO.1 TRC cloning plasmid through UNC core facility as part of mammalian gene knockdown consortium. .. Lentiviral infections were performed according to the manufacturer's instruction from System Biosciences (SBI).

    Article Title: The splicing activator DAZAP1 integrates splicing control into MEK/Erk regulated cell proliferation and migration
    Article Snippet: .. The knock down lines of DAZAP1 were made by cloning stable hairpin structure targeting DAZAP1 coding region (5′-CAGGGAATACTTCAAGAAGTT ) in pLKO.1 TRC cloning plasmid (Addgene). ..

    Article Title: Lysyl hydroxylase 2 induces a collagen cross-link switch in tumor stroma
    Article Snippet: Scrambled shRNA (Addgene plasmid #1864) was a gift from David Sabatini, Whitehead Institute, Cambridge, Massachusetts, USA ( 61 ). .. Murine LH2 shRNAs were constructed by inserting the hairpin sequences into the pLKO.1-TRC cloning plasmid (Addgene plasmid #10878), which was a gift from David Root, Broad Institute, Cambridge, Massachusetts, USA ( 62 ). .. Target sequences for murine LH2 were SH1 (5′-CTGTGAAGGTTCTTGGTCA-3′), SH3 (5′-CCGCAATGCTAGAGATATG-3′), and SH5 (5′-CGTGAACATGTCTCTGAAG-3′). pMD2.G (Addgene plasmid #12259) and psPAX2 (Addgene plasmid #12260), which were gifts from Didier Trono, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland, were used for lentiviral packaging as described below.

    Article Title: Supporting Information for circNEIL3 inhibits tumor metastasis through recruiting the E3 ubiquitin ligase Nedd4L to degrade YBX1
    Article Snippet: The full-length of the open reading frames of YBX1 and Nedd4L were amplified respectively by RT-PCR and cloned into the vector pCDH-CMV-MCS-EF1-Puro (System Biosciences). .. To construct YBX1 and Nedd4L knockdown plasmids, the annealed oligos targeting coding region were designed and respectively inserted into pLKO.1 TRC cloning plasmid (Addgene). ..

    Article Title: Lysyl hydroxylase 2 induces a collagen cross-link switch in tumor stroma
    Article Snippet: Scrambled shRNA (Addgene plasmid #1864) was a gift from David Sabatini, Whitehead Institute, Cambridge, Massachusetts, USA (61). .. Murine LH2 shRNAs were constructed by inserting the hairpin sequences into the pLKO.1-TRC cloning plasmid (Addgene plasmid #10878), which was a gift from David Root, Broad Institute, Cambridge, Massachusetts, USA (62). .. Target sequences for murine LH2 were SH1 (5′-CTGTGAAGGTTCTTGGTCA-3′), SH3 (5′-CCGCAATGCTAGAGATATG-3′), and SH5 (5′-CGTGAACATGTCTCTGAAG-3′). pMD2.G (Addgene plasmid #12259) and psPAX2 (Addgene plasmid #12260), which were gifts from Didier Trono, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland, were used for lentiviral packaging as described below.

    Plasmid Preparation:

    Article Title: An extensive program of periodic alternative splicing linked to cell cycle progression
    Article Snippet: The Myc-His-Ubiquitin expression vector is a gift from Dr. Gary Johnson’s lab, and the Histone H2B-GFP expression vector is gift from Dr. Angelique Whitehurst’s lab. Plasmid transfections were performed using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol. .. The lentiviral vectors of shRNAs were obtained from Addgene in pLKO.1 TRC cloning plasmid through UNC core facility as part of mammalian gene knockdown consortium. .. Lentiviral infections were performed according to the manufacturer's instruction from System Biosciences (SBI).

    Article Title: The splicing activator DAZAP1 integrates splicing control into MEK/Erk regulated cell proliferation and migration
    Article Snippet: .. The knock down lines of DAZAP1 were made by cloning stable hairpin structure targeting DAZAP1 coding region (5′-CAGGGAATACTTCAAGAAGTT ) in pLKO.1 TRC cloning plasmid (Addgene). ..

    Article Title: Lysyl hydroxylase 2 induces a collagen cross-link switch in tumor stroma
    Article Snippet: Scrambled shRNA (Addgene plasmid #1864) was a gift from David Sabatini, Whitehead Institute, Cambridge, Massachusetts, USA ( 61 ). .. Murine LH2 shRNAs were constructed by inserting the hairpin sequences into the pLKO.1-TRC cloning plasmid (Addgene plasmid #10878), which was a gift from David Root, Broad Institute, Cambridge, Massachusetts, USA ( 62 ). .. Target sequences for murine LH2 were SH1 (5′-CTGTGAAGGTTCTTGGTCA-3′), SH3 (5′-CCGCAATGCTAGAGATATG-3′), and SH5 (5′-CGTGAACATGTCTCTGAAG-3′). pMD2.G (Addgene plasmid #12259) and psPAX2 (Addgene plasmid #12260), which were gifts from Didier Trono, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland, were used for lentiviral packaging as described below.

    Article Title: Supporting Information for circNEIL3 inhibits tumor metastasis through recruiting the E3 ubiquitin ligase Nedd4L to degrade YBX1
    Article Snippet: The full-length of the open reading frames of YBX1 and Nedd4L were amplified respectively by RT-PCR and cloned into the vector pCDH-CMV-MCS-EF1-Puro (System Biosciences). .. To construct YBX1 and Nedd4L knockdown plasmids, the annealed oligos targeting coding region were designed and respectively inserted into pLKO.1 TRC cloning plasmid (Addgene). ..

    Article Title: Lysyl hydroxylase 2 induces a collagen cross-link switch in tumor stroma
    Article Snippet: Scrambled shRNA (Addgene plasmid #1864) was a gift from David Sabatini, Whitehead Institute, Cambridge, Massachusetts, USA (61). .. Murine LH2 shRNAs were constructed by inserting the hairpin sequences into the pLKO.1-TRC cloning plasmid (Addgene plasmid #10878), which was a gift from David Root, Broad Institute, Cambridge, Massachusetts, USA (62). .. Target sequences for murine LH2 were SH1 (5′-CTGTGAAGGTTCTTGGTCA-3′), SH3 (5′-CCGCAATGCTAGAGATATG-3′), and SH5 (5′-CGTGAACATGTCTCTGAAG-3′). pMD2.G (Addgene plasmid #12259) and psPAX2 (Addgene plasmid #12260), which were gifts from Didier Trono, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland, were used for lentiviral packaging as described below.

    Knockdown:

    Article Title: An extensive program of periodic alternative splicing linked to cell cycle progression
    Article Snippet: The Myc-His-Ubiquitin expression vector is a gift from Dr. Gary Johnson’s lab, and the Histone H2B-GFP expression vector is gift from Dr. Angelique Whitehurst’s lab. Plasmid transfections were performed using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol. .. The lentiviral vectors of shRNAs were obtained from Addgene in pLKO.1 TRC cloning plasmid through UNC core facility as part of mammalian gene knockdown consortium. .. Lentiviral infections were performed according to the manufacturer's instruction from System Biosciences (SBI).

    Article Title: The splicing activator DAZAP1 integrates splicing control into MEK/Erk regulated cell proliferation and migration
    Article Snippet: .. The knock down lines of DAZAP1 were made by cloning stable hairpin structure targeting DAZAP1 coding region (5′-CAGGGAATACTTCAAGAAGTT ) in pLKO.1 TRC cloning plasmid (Addgene). ..

    Article Title: Supporting Information for circNEIL3 inhibits tumor metastasis through recruiting the E3 ubiquitin ligase Nedd4L to degrade YBX1
    Article Snippet: The full-length of the open reading frames of YBX1 and Nedd4L were amplified respectively by RT-PCR and cloned into the vector pCDH-CMV-MCS-EF1-Puro (System Biosciences). .. To construct YBX1 and Nedd4L knockdown plasmids, the annealed oligos targeting coding region were designed and respectively inserted into pLKO.1 TRC cloning plasmid (Addgene). ..

    Construct:

    Article Title: Lysyl hydroxylase 2 induces a collagen cross-link switch in tumor stroma
    Article Snippet: Scrambled shRNA (Addgene plasmid #1864) was a gift from David Sabatini, Whitehead Institute, Cambridge, Massachusetts, USA ( 61 ). .. Murine LH2 shRNAs were constructed by inserting the hairpin sequences into the pLKO.1-TRC cloning plasmid (Addgene plasmid #10878), which was a gift from David Root, Broad Institute, Cambridge, Massachusetts, USA ( 62 ). .. Target sequences for murine LH2 were SH1 (5′-CTGTGAAGGTTCTTGGTCA-3′), SH3 (5′-CCGCAATGCTAGAGATATG-3′), and SH5 (5′-CGTGAACATGTCTCTGAAG-3′). pMD2.G (Addgene plasmid #12259) and psPAX2 (Addgene plasmid #12260), which were gifts from Didier Trono, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland, were used for lentiviral packaging as described below.

    Article Title: Supporting Information for circNEIL3 inhibits tumor metastasis through recruiting the E3 ubiquitin ligase Nedd4L to degrade YBX1
    Article Snippet: The full-length of the open reading frames of YBX1 and Nedd4L were amplified respectively by RT-PCR and cloned into the vector pCDH-CMV-MCS-EF1-Puro (System Biosciences). .. To construct YBX1 and Nedd4L knockdown plasmids, the annealed oligos targeting coding region were designed and respectively inserted into pLKO.1 TRC cloning plasmid (Addgene). ..

    Article Title: Lysyl hydroxylase 2 induces a collagen cross-link switch in tumor stroma
    Article Snippet: Scrambled shRNA (Addgene plasmid #1864) was a gift from David Sabatini, Whitehead Institute, Cambridge, Massachusetts, USA (61). .. Murine LH2 shRNAs were constructed by inserting the hairpin sequences into the pLKO.1-TRC cloning plasmid (Addgene plasmid #10878), which was a gift from David Root, Broad Institute, Cambridge, Massachusetts, USA (62). .. Target sequences for murine LH2 were SH1 (5′-CTGTGAAGGTTCTTGGTCA-3′), SH3 (5′-CCGCAATGCTAGAGATATG-3′), and SH5 (5′-CGTGAACATGTCTCTGAAG-3′). pMD2.G (Addgene plasmid #12259) and psPAX2 (Addgene plasmid #12260), which were gifts from Didier Trono, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland, were used for lentiviral packaging as described below.



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