plko 1 trc (Addgene inc)
96
Structured Review
Addgene inc
plko 1 trc
Plko 1 Trc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1296 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plko+1+trc+cloning+plasmid/pLKO%2E1+-+TRC+cloning+vector+(Plasmid+%2310878)/pmc12898032-213-8-19
Average 96 stars, based on 1296 article reviews
Plko 1 Trc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1296 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plko+1+trc+cloning+plasmid/pLKO%2E1+-+TRC+cloning+vector+(Plasmid+%2310878)/pmc12898032-213-8-19
Average 96 stars, based on 1296 article reviews
plko 1 trc - by Bioz Stars,
2026-09
96/100 stars
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Cloning:Article Title: An extensive program of periodic alternative splicing linked to cell cycle progression Article Snippet: The Myc-His-Ubiquitin expression vector is a gift from Dr. Gary Johnson’s lab, and the Histone H2B-GFP expression vector is gift from Dr. Angelique Whitehurst’s lab. Plasmid transfections were performed using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol. .. The lentiviral vectors of shRNAs were obtained from Addgene in Article Title: The splicing activator DAZAP1 integrates splicing control into MEK/Erk regulated cell proliferation and migration Article Snippet: .. The knock down lines of DAZAP1 were made by cloning stable hairpin structure targeting DAZAP1 coding region (5′-CAGGGAATACTTCAAGAAGTT ) in Article Title: Lysyl hydroxylase 2 induces a collagen cross-link switch in tumor stroma Article Snippet: Scrambled shRNA (Addgene plasmid #1864) was a gift from David Sabatini, Whitehead Institute, Cambridge, Massachusetts, USA ( 61 ). .. Murine LH2 shRNAs were constructed by inserting the hairpin sequences into the Article Title: Supporting Information for circNEIL3 inhibits tumor metastasis through recruiting the E3 ubiquitin ligase Nedd4L to degrade YBX1 Article Snippet: The full-length of the open reading frames of YBX1 and Nedd4L were amplified respectively by RT-PCR and cloned into the vector pCDH-CMV-MCS-EF1-Puro (System Biosciences). .. To construct YBX1 and Nedd4L knockdown plasmids, the annealed oligos targeting coding region were designed and respectively inserted into Article Title: Lysyl hydroxylase 2 induces a collagen cross-link switch in tumor stroma Article Snippet: Scrambled shRNA (Addgene plasmid #1864) was a gift from David Sabatini, Whitehead Institute, Cambridge, Massachusetts, USA (61). .. Murine LH2 shRNAs were constructed by inserting the hairpin sequences into the Plasmid Preparation:Article Title: An extensive program of periodic alternative splicing linked to cell cycle progression Article Snippet: The Myc-His-Ubiquitin expression vector is a gift from Dr. Gary Johnson’s lab, and the Histone H2B-GFP expression vector is gift from Dr. Angelique Whitehurst’s lab. Plasmid transfections were performed using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol. .. The lentiviral vectors of shRNAs were obtained from Addgene in Article Title: The splicing activator DAZAP1 integrates splicing control into MEK/Erk regulated cell proliferation and migration Article Snippet: .. The knock down lines of DAZAP1 were made by cloning stable hairpin structure targeting DAZAP1 coding region (5′-CAGGGAATACTTCAAGAAGTT ) in Article Title: Lysyl hydroxylase 2 induces a collagen cross-link switch in tumor stroma Article Snippet: Scrambled shRNA (Addgene plasmid #1864) was a gift from David Sabatini, Whitehead Institute, Cambridge, Massachusetts, USA ( 61 ). .. Murine LH2 shRNAs were constructed by inserting the hairpin sequences into the Article Title: Supporting Information for circNEIL3 inhibits tumor metastasis through recruiting the E3 ubiquitin ligase Nedd4L to degrade YBX1 Article Snippet: The full-length of the open reading frames of YBX1 and Nedd4L were amplified respectively by RT-PCR and cloned into the vector pCDH-CMV-MCS-EF1-Puro (System Biosciences). .. To construct YBX1 and Nedd4L knockdown plasmids, the annealed oligos targeting coding region were designed and respectively inserted into Article Title: Lysyl hydroxylase 2 induces a collagen cross-link switch in tumor stroma Article Snippet: Scrambled shRNA (Addgene plasmid #1864) was a gift from David Sabatini, Whitehead Institute, Cambridge, Massachusetts, USA (61). .. Murine LH2 shRNAs were constructed by inserting the hairpin sequences into the Knockdown:Article Title: An extensive program of periodic alternative splicing linked to cell cycle progression Article Snippet: The Myc-His-Ubiquitin expression vector is a gift from Dr. Gary Johnson’s lab, and the Histone H2B-GFP expression vector is gift from Dr. Angelique Whitehurst’s lab. Plasmid transfections were performed using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol. .. The lentiviral vectors of shRNAs were obtained from Addgene in Article Title: The splicing activator DAZAP1 integrates splicing control into MEK/Erk regulated cell proliferation and migration Article Snippet: .. The knock down lines of DAZAP1 were made by cloning stable hairpin structure targeting DAZAP1 coding region (5′-CAGGGAATACTTCAAGAAGTT ) in Article Title: Supporting Information for circNEIL3 inhibits tumor metastasis through recruiting the E3 ubiquitin ligase Nedd4L to degrade YBX1 Article Snippet: The full-length of the open reading frames of YBX1 and Nedd4L were amplified respectively by RT-PCR and cloned into the vector pCDH-CMV-MCS-EF1-Puro (System Biosciences). .. To construct YBX1 and Nedd4L knockdown plasmids, the annealed oligos targeting coding region were designed and respectively inserted into Construct:Article Title: Lysyl hydroxylase 2 induces a collagen cross-link switch in tumor stroma Article Snippet: Scrambled shRNA (Addgene plasmid #1864) was a gift from David Sabatini, Whitehead Institute, Cambridge, Massachusetts, USA ( 61 ). .. Murine LH2 shRNAs were constructed by inserting the hairpin sequences into the Article Title: Supporting Information for circNEIL3 inhibits tumor metastasis through recruiting the E3 ubiquitin ligase Nedd4L to degrade YBX1 Article Snippet: The full-length of the open reading frames of YBX1 and Nedd4L were amplified respectively by RT-PCR and cloned into the vector pCDH-CMV-MCS-EF1-Puro (System Biosciences). .. To construct YBX1 and Nedd4L knockdown plasmids, the annealed oligos targeting coding region were designed and respectively inserted into Article Title: Lysyl hydroxylase 2 induces a collagen cross-link switch in tumor stroma Article Snippet: Scrambled shRNA (Addgene plasmid #1864) was a gift from David Sabatini, Whitehead Institute, Cambridge, Massachusetts, USA (61). .. Murine LH2 shRNAs were constructed by inserting the hairpin sequences into the |